Biochem/physiol Actions

Target IC₅₀: Does not inhibit PI 3-kinase even at a concentration of 100 µM

Reversible: no

Product does not compete with ATP.

Primary TargetNegative control for the PI 3-kinase inhibitor LY 294002

Cell permeable: yes

General description

A cell-permeable, negative control for the phosphatidylinositol-3-kinase (PI-3 kinase) inhibitor, LY 294002 (Cat. No. 440202). Contains a single atom substitution in the morpholine ring compared to LY 294002. Does not affect PI-3 kinase activity even at concentrations ≥100 µM.

A cell-permeable negative control for the PI 3-kinase inhibitor LY 294002 (Cat. No. 440202). Contains a single atom substitution in the morpholine ring compared to LY 294002. Does not inhibit PI 3-kinase even at a concentration of 100 µM. Shown to bind to the same site as I-BET (Cat. no. 401010) and reversibly inhibit BET bromodomain proteins BRD2, BRD3 & BRD4 (IC₅₀ ~10 µM).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Dittman, et al. 2014. ACS Chem Biol.9, 495.Ding, J., et al. 1995. J. Biol. Chem.270, 11684.Vlahos, C.J., et al. 1994. J. Biol. Chem. 269, 5241.

Packaging

1 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.r>

Warning

Toxicity: Standard Handling (A)